I am a biologist by profession. While shooting insects for my research, I use a laboratory binocular microscope (with no attachments). I have been using Canon PowerShot SX120IS to shoot the same, but the images seem underexposed. I have tried to improve the light source. When I see the image on the LCD display, it looks alright, but as soon as click the button to capture, the final image still looks underexposed. Please tell how to solve this problem.
Omkar Mandke, Pune
Since the LCD shows you a properly exposed image, you could be using the wrong exposure settings. Typically, whether you prefer to use the Program Mode or wish to experiment with the Manual mode, you must always ensure that the camera’s meter shows an exposure value of 0EV. If it indicates +EV or –EV, then make sure you adjust the settings accordingly. You can also use the histogram in your camera to judge exposure accurately. Also, since you are shooting inside the laboratory, you may require a slightly slow shutterspeed, for which you will need to use a tripod. Lastly, if possible, take the microscope closer to a source of light, like a window or a table lamp, to ensure that the subject is properly lit.